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Tuesday 26 March 2019

DNA Extraction MWHAHAHHHAHHAHAHA

Aim: To extract the DNA of the kiwifruit so we can observe it

Method
  1. Using a spatula,  scoop 1/2 of the kiwifruit into a bag and add 25ml water + a pinch pf salt.
  2. Mush until a thick soup.
  3. Pour over cloth into funnel into beaker
  4. Pour liquid into test tuber. Add dish washing liquid + swirl. Allow to sit for 5 minutes.
  5. Tilt test tuber on 45 degree angle and slowly pour 10ml of ethanol down the side. 
  6. Allow to sit.
Results
Hopefully after you allow it to sit your test tube should look like this:

The jelly fish egg looking stuff on top is the kiwifruit's DNA. If it doesn't look like this then you might've not mushed the kiwifruit enough. If it has big clumps then not much of the kiwifruit will make it through the cloth.

Discussion:
We used water to help break and liquefy the kiwifruit which would allow it to pass through the cloth. Salt allowed the DNA clump together while ethanol helps the DNA float to the top. The reason the DNA is bubbly like that is because the detergent that creates air bubbles that make it easier to see.

Conclusion
We were able to separate the DNA successfully. I also learnt what would happen if someone drank ethanol (because it's stronger than stomach acid you would get a burning feeling inside and then most likely die).
Photos
 




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